Varsha Komalla

4 Questions 5 Answers 0 Followers

Questions related from Varsha Komalla

How can you ensure that the drug is completely separated from liposomes?

After doing either sephadex column or ultrafiltration. How do you ensure that entire unentrapped drug is removed from liposomes? Will there be any extra peak in DLS?

11 January 2018 1,427 6 View

Does any know why we get negative values in ELISA?

I performed ELISA for liposomes and got negative values in untreated control and few treatment groups. But positive control, negative control and other treatment groups are all positive. Note: I...

25 October 2017 6,992 11 View

All the papers I read have shown endocytosis uptake of liposomes. Why dont we study other mechanistic uptakes?

there are four different mechanisms for liposome uptake-adsorption, fusion, endocytosis and lipid exchange. But why do we only study endocytosis mechanism while exploring cell uptake mechanisms of...

02 October 2017 2,677 3 View

How to reduce interference of phospholipids and drugs in HPLC?

Hi, I am preparing liposomes with drug loaded inside them. I would like to quantify the drug inside them through HPLC. There is an established HPLC method for that drug.Somehow I am not able to...

12 July 2017 2,222 4 View