How to reduce cell death in definitive endoderm stage of iPSCs hepatic differentiation?

24 February 2024 0 7K Report

I tried several approaches, including CHIR and Activin A for one day and Activin only on the second and third day switching towards progenitor media containing 1% DMSO.

I also tried FBS and B27 in the DE media, However, cell death was massive in the second and third days.

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