I read this paper on determining the viability of cells by BioTimer assay1 and improvement of the same by another paper 2, but I am still not very clear upon the methodology. Could someone please elaborate on the same? A noble method also works. I need to determine the number of viable bacteria in the biofilm sample.
1- BioTimer Assay, a new method for counting Staphylococcus spp. in biofilm without sample manipulation applied to evaluate antibiotic susceptibility of biofilm. Fabrizio Pantanella a, Piera Valenti a, Alessandra Frioni a, Tiziana Natalizi a, Luana Coltella b, Francesca Berlutt (2008)
2- A Method for Quantitative Determination of Biofilm Viability. Ken Welch , Yanling Cai and Maria Strømme (2012)
you have these papers...
did you do livedead staining, also try alamar blue method.
are you looking for a protocol?
Dear Rajat
I recommend you to read this paper : Article Critical review on biofilm methods
hi
we have a different methods but i can recommend this article for you:
http://dx.doi.org/10.1016/j.micpath.2015.11.031
@Jaroslaw Krol Yes, I did live/dead staining. For viability assay I understood the theory but I don't really understand the practical steps. Please just explain the practical steps.
You can do a live/ dead staining
then microscopy followed by image analysis using ImageJ
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