How to quantify results in HPLC UV detector or any formula applying?

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While performing MD simulation, is it possible to add other ions while solvating in addition to 0.15 M of NaCl and MgCl2?

Can an MD simulation be performed by adding other salts by varying their concentration inside the box?

01 May 2023 9,230 3 View

How can be the protein immobilized s-CM5 sensor chip stored?

I have stored the cm5 chip in 50 mL of HBS-EP buffer pH 7.0 at 4°C... which buffer can be used for long-term storage of the chip?

10 April 2023 4,319 2 View

In a MD simulation, a protein complex with > 600 amino acid can have a RMSD over 1 nm (10 Angstorm)?

MD simulation of a protein complex with more than 600 AA residues shows an average rmsd between 1-1.5 nm. can the protein complex can be considered stable? Besides sometimes i see a jump in the...

06 November 2022 6,302 4 View

Is it possible to indulge a dimeric protein (Target) into docking against another protein (Bound to a metal ion) using HADDOCK server?

I have been using CLUSPro 2.0 for protein-protein docking, but it does not take the metal ion into consideration because it selectively takes the protein chain. In contrast, HADDOCK considers the...

06 June 2022 3,285 4 View

Is there any way/tools to interepret the molecular dynamics simulation results beside XmGrace?

I have done MD simulation and want to interpret and plot the data from the files. As my PC did not have enough specs for Dual boot OS because the XmGrace tool is supported only on Linux. Though, I...

25 April 2022 4,254 3 View

Whether ethical committe (IAEC) approval is needed for samples procured from butcher shop?

I am devising a study to evaluate the invitro anti- resorptive effect of my sample in bovine bone procured from butcher shops/markets. Though there are studies in which the samples from...

20 September 2021 4,259 6 View

How to find the amino acids which are located in the particular protein binding site?

As i am working on protein-ligand docking, i want to input the x,y,z coordinates in autodock and to do that, i need the coordinates of aminoacids present in the binding site. So, PLease help me out.

03 January 2021 4,099 4 View

Importance of distance education?

Distance learning is a learning method in which students are not required to be physically present in a classroom setting or school.

25 December 2020 8,704 10 View

Axia piston pump PHM Literature Review- Collaboration ?

Hi All , I am planning to write a literature review on axial piston pumps condition monitoring. Any researchers would like to collaborate on this ? With regards Bubathi

22 December 2020 5,850 3 View

Farming is an non profitable occupation ? If agree / disagee reasons?

Some of the problems faced by farmers..... 1. Poor marketing channel 2. Farmers not getting remunerate price for the own produce 3. Lack of bargaining power (someone fix the price) 4. Monsoon...

30 November 2020 3,819 4 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Gunawan Indrayanto

Hi, I attach an application note, please read pages 27-36, I hope it can answer your question, best regards

Muruganantham Paramasivam

Thank you very much sir. I will can apply for my research work.

Bujji Reddy Kanchi

(Sample area/Standard area) x (Standard concentration in mg per ml/sample concentration in mg per ml) x Purity of the standard in %