How to quantify protein in tissue by immunofluorescence?

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Anurag Nishad

Hi Qianjiang Chen ,

If you could elaborate on your problem as well as give some images, I might be able to help you.

Dinesh Raj Pant

Hello Qianjiang Chen,

You need to delineate the region using ImageJ software alongside the reference protein, allowing it to analyze and measure the color intensity generated.

Hope it will work.

Regards

Hazem Almhanna

Use image J, it is very nice and accurate, but you need to normalize your data after maesuring the expression of your protein.