I'm trying to find the most simple conditions to quantify nicotine in liquid samples (e-cigarrettes liquid). I'd like to try with a standard 150 mm C18 column. Any suggestions about the mobile phase composition will be appreciated. We have a DAD detector therefore I expect to be able to check the peak purity easily.
Hello, you can begin with 80% acetonitrile (or methanol) and 20% water with 0.2% formic acid. If retention time is too short you then decrease acetonitrile. Good luck.
In addition to this question, you can use ACN: 0.1%H3PO4 (10:90), C-18 with flow=1.0ml/min
Gudluck
:)
Thanks for all your answers. Actually, i've tried with a combination of 20 mM AcONH4 pH 8,7(A):ACN(B)1 ml/min. I've applied a gradient from 100%A to 50%A in 10 minutes. It's not a pretty peak (poor symmetry - long tail) but overall, enough for quantitation, and it resolved all my sample peaks. It's a basic mobile phase and i'm using a typical C18 column. I'm not sure if i',m damaging it, but the retention times are repeating ok, and also the pressure throught the cycle in different injections...
Do you think is it possible to perform this separation in acidic mobile phase?. I didn't try it cause i expected more success in alkaline media.
Thanks 4 all
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