Salvador Valero

7 Questions 17 Answers 0 Followers

Questions related from Salvador Valero

How to separate glucose from the matrix prior to HPLC-ELSD aminoglucoside analysis?

Hi, we're trying to determine two amiglucosides antibiotics and one aminoacid in a powder mix whose principal ingredient is glucose: 60 % w/w. The antibiotics are amikazine and apramycin, the...

23 May 2024 5,685 0 View

Best methodology for performing fast anion chromatography in tap waters?

Hello, in our lab we've been using anion chromatography with conductimetric detection for the determination of 6 anions in tap and natural waters, namely Fluoride, Chloride, Nitrite, Nitrate,...

04 February 2019 5,157 2 View

What's this peak?

Hi, i've injected 5 microliters of pure CHCl3 in a 250 mm C18 column, with ACN:H2O 80:20 as solvent, 1,0 ml/min. Something appears at 3,72 min, absorbing strongly at 210 nm. Is the CHCl3 absorbing...

21 December 2017 8,909 17 View

What do you think about this peak tailing?

Hi, the attached graph shows consecutive injections of analytical standards of dexamethasone sodium phosphate.(20-200 ppm) dissolved in the eluent. Conditions used: Eluent: Phosphoric acid 0,1 % +...

22 March 2017 8,142 13 View

Anyone familiar with VF-5ms column with methanol as solvent?

Hi, we'd like to analyze an SPE extract whose solvent is methanol, with a GC-MS/MS (ion trap), split/splitless injector, and this column. The supplier told us about not to inject in this column...

18 February 2015 6,914 3 View

How to quantify nicotine by HPLC-DAD?

I'm trying to find the most simple conditions to quantify nicotine in liquid samples (e-cigarrettes liquid). I'd like to try with a standard 150 mm C18 column. Any suggestions about the mobile...

24 December 2013 617 3 View

ICP-OES recommendation

We're actually planning to acquire an ICP-OES for the analysis of metals in tap water, waste waters, sludges, and other environmental matrices. We're especially worried about the hydride-forming...

22 March 2013 296 12 View