Does anyone know of a predictable relationship beween surface gravity and atmospheric composition?

The attached file demonstrates that there is a predictable relationship between the structural density of a terrestrial body and its none-rotating surface gravity. Using Newton's gravity law of...

26 February 2021 2,317 3 View

What is the chemical composition of chlorellin?

The chlorellin is the chemical compound which is first extracted from Chlorella vulgaris by Pratt in 1944. Spoehr in 1949 wrote that it is a mixture between fatty-acids and hyrdocarbons and its...

25 January 2021 8,538 1 View

Is it possible to dynamically adjust the data rate of a wireless node using TSCH protocol in Link Layer?

I'm currently learning about rate-based congestion control for industrial wireless sensor network. Suppose there is a network consisting of several sensor nodes and one coordinator node connected...

12 November 2020 373 2 View

How long can PBMCs survive outside of an incubator in R-10 culturing medium?

I have been running ELISPOTS with not much success recently due to high levels of background. After testing everything from the DMSO used in peptide stimulation to the FBS in the culturing medium,...

15 October 2020 849 3 View

How dissolve Hyaluronic acid with consistent viscosity?

I'm trying to dissolve HA in PBS or cell media, 20 mg/ml, but gets inconsistent viscosity on my solutions. Some samples are very viscous while others are very liquid. The HA functionalized with a...

30 June 2020 4,075 3 View

What does it mean if an anaplastic thyroid cancer has no mutations or fusions in a comprehensive PCR analysis?

The tumor has p53 possitivity, but a comprensive PCR analysis showed no mutations in tp53.

30 March 2020 884 3 View

What does a Negative Phi and Cramers V coefficients imply?

From the way we define the phi and Cramers V and from my theoretical knowledge, I expect Phi coefficient and Cramer's V to bare positive values. In a recent analysis using SPSS 22.0, Negative...

22 March 2020 4,064 6 View

How do I obtain Total Faradaic effeciency close to 100%?

After CO2 Electrochemical reduction on 6 samples, i obtained both gaseous and liquid products from GC-MS and NMR respectively. i quantified the liquid products by integrating each peak. for...

14 December 2019 5,479 4 View

Entrepreneurial Orientation Measurement Results?

Hello, I am looking for a review of EO measurement results via the Miller/Covin Slevin scale. I mean the actual values for innovativeness, risk and proactiveness. There are a number of papers who...

18 November 2019 8,474 3 View

Human-centered Design Questionnaire?

Hello, I'm searching for a [validated] Human-centered Design questionnaire. I've been searching in the Industrial Design and Human-computer Interaction literature, but have come up short. The...

26 October 2018 4,853 4 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

Proteins not stacking/ entering in a native PAGE. Can anyone help?

Hi. I am trying to study a biotin modification of my target protein in a crude cell extract and am interested to determine how much of my protein in getting biotinylated. After biotinylating my...

15 January 2021 2,214 4 View

How to bind streptavidin to the surface of acrylamide?

We are fabricating acrylamide beads as probe substrate. How can we make streptavidin bind to the surface of the acrylamide? Thanks!

09 December 2020 8,141 7 View

How to solve the problem with protein getting stuck in the wells during electrophoresis for EMSA?

26 November 2020 6,094 3 View

mScarlet Elisa Detection?

I've been trying to detect mScarlet protein purified from E. coli via an ELISA assay. My protein has two tags: an N-terminal Strep-tag and a C-terminal His-tag. To do the ELISA I've used...

20 September 2020 730 2 View

Best way to add streptavidin onto flat surface?

I will be adding streptavidin onto the surface of a graphene coated glass slide; thereafter, I will be analyzing with a MALDI-TOF. I was wondering the best way to go about this? I was thinking of...

07 September 2020 5,902 4 View

Negative Inhibition in Confirmatory ADA assay?

I am developing an Anti-Drug Antibody assay and keep getting a negative percent inhibition (negative control sample results are higher in confirmatory than in screening format) when running...

19 August 2020 6,658 1 View

How can I break biotin-streptavidin bond and separate them?

I am working with a florescent compound that is biotinylated. When strepdavidin is present in the system, the binding of the protein with the compound quenches the florescence. I need to perform...

16 August 2020 6,937 8 View