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I would like to purify and isolate secreted proteins with a biotin tag for massspec analysis from cell culture medium using streptavidin beads. Does anybody have a protocol? Many thanks
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I am trying to do the 3D PCR and realized that the amount of adding DMSO or Betaine influences the outcome of the second nested PCR. Could anyone send me a protocol for the first and second PCR...
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I would like to address whether my genetically engineered human AD293 cells are more or less attacked by T-cells. Is there a human T-cell line that can be used for a cytolysis assay?
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Hi I have generated SV40 immortalized mouse fibroblasts from the ear and would like to use them for xenograft experiments in mice. Does anybody have literature about this experimental setting...
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