How to do data analysis of a split plot design experiment with an extra control treatment outside the design but in the same field?

In an split plot experiment, 4 methods of Nitrogen(N) application was assigned in main plot and 3 doses of N in subplots and replicated thrice. Along with it, in the same field, No N control...

20 September 2020 9,944 2 View

Is it mandatory to detect target protein and control (actin or tubulin) in the same membrane?

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30 May 2019 10,014 2 View

Hello Friends, How much % of impurity need to be generated in forced degaradation (FDS) of Biomolecules?

Bio molecules are less stable compared to Pharma drugs. Since proteins are notorious for stability many impurities gets generated on the stability. If impurities are not available, we need to...

22 March 2018 6,414 3 View

How to separate G and D' peaks of highly defective Graphene or vertical graphene nanowalls in Raman spectroscopy.

21 June 2017 3,672 2 View

How to purify all the nucleic acid (DNA and RNA) associated protein from bacteria?

22 September 2016 3,836 2 View

Can anyone help with Ansys 14.1 transient full mode analysis?

06 July 2015 9,580 3 View

Why core shell quantum dots is not dissolving in water or ethanol?

17 February 2015 4,963 3 View

What is the optimum irreproducible retention time of the peak in HPLC?

I am doing reverse phase HPLC using C18. Keeping the flow rate and composition of solvent constant, I should get my target peak at 14 min, but I am getting the peak sometime at 14 minutes sometime...

27 June 2014 4,361 4 View

What is the role of copper in anaerobic metabolism?

I want to know the role of copper in anaerobic metabolism and also I like to know the interaction between the nanoparticle and bacteria and how nanoparticle inhibit / enhance bacterial growth.

14 May 2014 9,429 2 View

Can anyone help with this Gaussian error message- "write error in NtrEx1"?

An error message " write error in NtrEx1" is showing when I was doing optimization and frequency calculation in Gaussian 09. Can anyone help me?

04 May 2014 8,930 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View