16 Questions 24 Answers 0 Followers
Questions related from Anh Hoang Le
I'm just wondering if I need to change the media of my cells that are culturing in 5% O2 (hypoxic condition) in a hypoxic chamber aka a hypoxic glove box? Or can I just quickly do this in a normal...
28 March 2024 2,433 4 View
So during meiosis, we know that homologous chromosomes (one from dad, one from mom) pair up and for a tetrad for then division and result in haploid gametes. But during mitosis, we know that...
06 August 2022 7,087 0 View
Hello, some journal asks for video in MP4 format but my mac only has AVI. Does anybody know any way to convert these files to MP4 without or with minimum resolution loss? Thank you very much
19 April 2021 6,821 2 View
I have a question regarding which residues should I mutagenise into. By using bioinformatics method, I have identified some putative amino acid residues (Isoleucine, Methionine and Leucine) that...
03 September 2018 9,742 1 View
At the moment, I'm trying to analyse a difficult set of data that I've been thinking but really don't know how to do it. So basically, i have two populations of cells and I notice one of them...
20 February 2018 3,767 2 View
Hello, I'm very much aware of the difference between cell migration in 2D vs 3D. As many papers and PIs have always mentioned this. However, just how difference this is, i dont really have an...
27 June 2017 8,091 11 View
I'm just wondering if anyone has any experience about the non-specific binding of GFP to the cytoskeleton, particularly actin fibers? Is it possible for the GFP protein to interact with actin...
02 February 2017 8,080 4 View
I'm having a problem with gelatin coating. My gelatin for some reason solidifies prematurely and when i look under the microscope, i can see a lot of lumps. I tried many different ways from...
20 December 2016 592 6 View
I'm doing an invadopodia assay, and from what I found on the literature, people use x63 magnification of the confocal microscope to find a specific area, image it and use ImageJ to analyse the...
10 December 2016 6,385 9 View
So I designed some primers but the melting temperature is all above 70 degree. I know that this should not be done, but to meet all the criteria, there is no other way. My main primer is 21 base...
08 December 2016 6,735 4 View
Hello, I'm new to RNA extraction and RT-qPCR. At the moment, I'm doing a lot of RNA extraction. My first initial result was actually good with high concentrations of RNA and pure. This stops when...
20 September 2016 9,357 2 View
So we all know that humans cannot digest hair because "we don't have the enzyme needed to digest keratin which is the main component of hair". But I wonder, if this is true, then why can we...
13 May 2015 8,232 7 View
As we all know, with a linear chromosome, on the lagging strand (template 5'->3') of DNA replication, when the last piece of RNA primer at the 3' end is removed, the DNA cannot be extended and...
13 February 2015 390 10 View
I hope someone can clear this out for me. I find them a bit confusing and also a bit contradictory. Basically, we all know that Eo is the standard redox potential relative to the Standard Hydrogen...
10 February 2015 9,473 3 View
There seem to be two different concepts: one is the recombinant frequency and then there's crossover frequency. These two seem to be used interchangeably whenever someone asks but I think these...
01 January 1970 9,022 2 View
I've been making CRISPR knock-out cell lines of my protein of interest. Unfortunately, there is no commercially available antibody to check the presence of my protein. The only method I have been...
01 January 1970 3,746 3 View
