Abid Ali

4 Questions 12 Answers 0 Followers

Questions related from Abid Ali

What are good methods of synchronizing cancer cells in particularly in G1, S, G2/M stages ?

I am using MCF7 cell line which doubling time is greater than 30 hours, will the thymidine double blocks (block 1; 16 hr-->9 hr release-->block 2: 15 hr ) works better or serum starvation protocol?

05 May 2019 4,201 5 View

When we need to induce cells while doing a scratch assay?

I am working with inducible cell lines, I want to do scratch assay but I am not quite sure either to induce the cell a day before scratch assay so that we can clearly see the affect of our desire...

11 November 2018 4,095 4 View

Does a same DNA construct used for transfection of different cell lines, will affect protein size slightly or not?

I have transfected two different cell lines with same DNA construct but there is a slight difference in the size of the same protein harvested from those different cell lines. Did anyone...

10 October 2018 3,334 2 View

How to prevent fungal contamination in culturing flask of leishmania in field?

We are facing the problem of contamination despite of using vacuumed tubes, even antibiotics and anti fungal drugs were also used in the media (RPMI1640 supplemented with 10% FBS .

05 May 2017 908 2 View