Actually,I have HT29 DOX treated petri dish in which large amount of cells seem to get detached and float in the medium after 24 hrs of treatment.Kindly tell me how to prevent detachment of the cells so that I may get adequate amount of cells at the end of the treatment.
Note: By DOX treatment,the cells die,but the dead cells after every 24 hrs of treatment should be moderate as I observed in earlier treatment.but in this case I can see above 60% cells floating in the medium and the medium color turns yellow.
Hi,
maybe it might help to coat the dish with a substrate for better adherance of the cells, such as poly-L-Lysine. We generally use it if we want to transfect cells that tend to detach and it works quite well.
So you just dilute it in PBS, put it into the dish, incubate for ~1h and wash the dish carefully twice with sterile water and make sure to aspirate the water properly in the end. If you like, you can do an additional washing step with the media of the cells. Then you can seed your cells as usual.
However, if the media turns yellow and cells start floating a lot, it might also only indicate a bacterial contamination of your dish.
Good luck,
Cindy
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