18 Questions 5 Answers 0 Followers
Questions related from Abdul Rouf
Dear Folks, Greetings! I would like to know: What are the optimal expression conditions and purification strategies to obtain soluble and functionally active recombinant toxins from E. coli, and...
21 July 2025 4,844 2 View
Dear Folks, Greetings! I would like to know how one can effectively purify proteins from bacterial culture (with Mol. wt. say, approx. 37 kDa) using Ni-NTA Econo column. Sharing any protocols...
07 February 2025 4,529 4 View
Hello everyone! Hope you are fine and doing well. I would like to know if someone has worked with HMGB1-Gluc construct recently and I would be highly grateful if he or she could forward some...
31 October 2022 4,847 0 View
Hello everyone, Greetings! Hope everyone is fine. I would like to confirm if someone has performed ChIP assay via. shortcut method and has got the output successfully. Kindly forward the protocol...
06 August 2022 9,029 0 View
Hello all, I would like to know the toxins which block cytokinesis without blocking nuclear fragmentation apart from Cytochalasin B.
16 February 2022 8,416 3 View
Hello Everyone, Greetings! Hope everyone is fine. CBMN assay involves Cytochalasin B which blocks cytokinesis by inhibiting actin filament formation which leads to micronuclei formation in the...
07 February 2022 4,453 1 View
Hello everyone, Greetings! Hope everyone is fine and in good health in the current Covid19 pandemic.I would like to know the difference between the Reverse Transcriptase-qPCR and normal realtime...
07 February 2022 2,937 5 View
Hello everyone, Greetings! If in a petridish,you have live cells mixed up with debris and dead cells and you wanna separate only live cells to cultivate them further or use them for other...
16 December 2019 8,600 5 View
Hello everyone! I am working with Human cancer cell lines. I can't find the gene sequences for MACF1 isoforms(homo sapiens). I just want to design primers for MACF1 isoforms to check the gene...
14 October 2019 1,385 2 View
Hello everyone, I would like to know about how you can remove the different kinds of debris which are observed in the petridishes/flasks which contain cells in suspended form in the growth...
01 June 2019 6,365 3 View
Hello everyone, I would like to know about the formation of spheroids (for cancer stem cells) in cancer cell lines using B27 supplement. If someone has succeeded in developing spheres in cancer...
30 May 2019 3,242 3 View
Hello everyone, I would like to know about the growth of SKOV3-mct cell line growth in DMEM medium supplemented with 10% FBS. Do the cells show slow growth or the cells die to a greater extent....
30 May 2019 8,076 0 View
If a researcher added 10ml of Absolute ethanol to 1g Progesterone(MW=314.46g/mol),then he/she again added approximately 51ml of ultrapure water to it.He/she complains that progesterone didnot get...
21 March 2019 1,869 4 View
Hello everyone,If someone is working with curcumin at present,then,kindly let me know about the storage process of curcumin stock solution,whether you store the 100mM curcumin stock solution at 4...
30 April 2016 3,938 2 View
Actually,I have HT29 DOX treated petri dish in which large amount of cells seem to get detached and float in the medium after 24 hrs of treatment.Kindly tell me how to prevent detachment of the...
24 February 2016 4,540 2 View
I checked so many protocols in which only CBB G-250 has been used so far.Kindly help me if we can use CBB R-250 also for protein estimation/BSA standard.
09 February 2016 9,167 1 View
I prepared 500ml of McCoy's Medium from the powder using autoclaved water and then filtered it using the filter pump.Then i kept 10ml of the same filtered medium for testing by adding 10% FBS and...
02 February 2016 3,891 3 View
Hello everyone! Hope you are doing good. Let's discuss together regarding the efficacy of Helicase over CRISPR/Cas9 and how far Helicase has reached to the market for its applications in geen editing?
01 January 1970 3,280 0 View
