Dear Anik,

In the case de P. oryzae, our reserach group performs the preservation of the isolates by the method of paper filters, collected with milk and carried in a jar with silica, maintained at 4 C.

I hope the method will work,

Sincerely,

Carlos

Dear Carlos,

Thanks for the information. Yes, I will also do that. That is normal. However, I want to have extra copies. That is why I want to store them in glycerol as well. But I do not know the procedure for Pyricularia.

By the way, which media do you use for getting higher sporulation?

i hope following link would help you.

http://www.environmental microbiology.de/pdf_files/Glycerolcultureslongtermstorage4jan2013.pdf

Dear Anik

I preserved P.oryzae in glycerol and kept in 4 degree, however some isolates can't survive after 2 years. I would suggest to preserve in paper filter.

Cheers,

Wanporn

Hello Khemmuk,

In which form you preserve the fungus? Is it a conidial suspension or piece of medium with mycelium?

Hi, check for these useful papers,

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=4&cad=rja&uact=8&ved=0ahUKEwiG9fnv_vzVAhWl7IMKHVm4CvUQFgg7MAM&url=https%3A%2F%2Fwww.researchgate.net%2Ffile.PostFileLoader.html%3Fid%3D581ccb7f96b7e42e0607c221%26assetKey%3DAS%253A424760111833088%25401478282110883&usg=AFQjCNFr7gOL_UfTpnCuwWOfE6H-w6Xhmw

https://link.springer.com/chapter/10.1007/978-94-017-2157-8_8