How to do Haplotype-based GWAS?

Hi, I have a SNP dataset containing 717045 SNPs. I want to a GWAS using Haplotype blocks. But I do not know first how to create haplotype SNP dataset for GWAS and also the criteria for creating...

04 May 2019 5,732 4 View

What is point of transformation if the effects does not change?

Hello, A question related to transformation. I have done a linear mixed model using the formula "trait~Iso+Host+Iso:Host+Replication+Iso:Replication+Host:Replication". It is a greenhouse...

04 May 2019 3,637 8 View

How to draw a scatter plot point with two colors in R studio?

Hi, I have a data set containing hybrid yield in one column, the cross combination of two parents in one other column and another variable in the other column. I want to make a scatter plot...

01 February 2019 362 6 View

Which software to use for trimming sanger sequenced data, making contigs and mapping to reference gene?

Hi, I have an amplicon of 1.6kb and I have sequenced it using forward and reverse primer. Now, I have to trim both the sequences and assemble those into one contiguous sequence. My goal is to...

08 September 2018 1,174 5 View

Can anyone give me a head start for doing GWAS?

I want to perform genome wide association mapping for plant disease resistance gene. Since, I am completely a beginner in this field, it would be really helpful if someone can give me some advice...

31 December 2017 2,012 5 View

How to preserve wheat blast fungus in glycerol for long term storage?

Hello, I want to preserve wheat blast fungus in glycerol for long term storage. However, I do not know the procedure for this particular fungus. What should be the concentration of glycerol? Do I...

07 August 2017 338 7 View

How to interpret non significant F-test for main effect (genotype), but significant pairwise comparison?

Hello, I am analyzing a data set based on the trait values under drought condition. The experiment was carried out on three separate drought environments. First I have done a single experiment...

05 June 2016 2,290 13 View

How to calculate grain yield according to plot size and dry weight?

Hello, in my experiment I have 3 locations in which two locations have the same plot size and the other location has a different plot size. Again in the two locations with same plot size the...

04 May 2016 6,521 4 View

How to deal with outliers?

Hello, I am doing data analysis with PLABSTAT, after the analysis I am getting information about the suspected outliers. So, how can I deal with it? Should I delete those values and regard the...

03 April 2016 1,035 7 View

How should I perform data analysis when there are different number of replications and traits measured across 2-3 locations?

I have the data of maize drought trials. There are 3 locations and 5 environmental conditions where the trails were done. Now, the problem is that the data are very unbalanced. There are different...

02 March 2016 4,004 6 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

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01 March 2021 3,905 5 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

How can I separate Gertrud Pfister's profile and the profile of Gerald Gems?

we are co-authors of several publications. But I am the only and single author of most of my publications which are based on my own research. How can I separate the profiles?

21 February 2021 4,582 3 View

Can I follow Researchers from Teaching and testing areas?

I want Researchers from Educational Measurement and Evaluation, relating to teaching, learning , academic performance and test validation

18 February 2021 4,757 13 View

What are the LAB methodologies (procedures, equipment etc) for CO2 enrichment experiments to study crop responses?

I am doing a proposal for a research entitled 'GRAIN YIELD AND QUALITY RESPONSES OF WHEAT GENOTYPES UNDER CO2 ENRICHMENT AND EXTREME EVENTS' I will be using climate chamber and I would like to...

18 February 2021 6,494 4 View

Why use both bead beating and enzymes (lysostaphin and lysozyme) for DNA extraction from sputum samples?

Hello, I have read in a few publications that to extract total DNA from sputum samples, both bead beating and enzymes are used to disrupt the cells. Bead beating is supposed to be efficient in...

18 February 2021 3,360 2 View