I am facing issues in getting the standard curve of APTES in ninhydrin test. Should i take direct APTES(0.2ml, 0.4 ml.....1 ml) in various concentration or i need to prepare stock solution for APTES?
I intend to model a water-chloroform interface with some polymer chains. How can I calculate the number of atoms and the size of the simulation box by knowing the density for the MD simulation?...
05 May 2024 1,701 0 View
Hi, I am currently performing NVT equilibration of i-pma in a water-CCl4 box. Four moles of pre-equilibrated pma with a length of 30 units were randomly inserted into the pre-equilibrated...
10 April 2024 4,291 0 View
Hello, I'm conducting a beta galactosidase staining assay for my fibroblasts using X-Gal. I dissolve X-Gal (stock 50mg/ml prepared in DMSO) in the staining buffer at a final concentration of...
25 March 2024 8,799 3 View
The absence of Ocean Island Basalts (OIBs) in the Archean rock records is commonly attributed to the higher melting degrees associated with mantle plumes. However, this explanation raises...
14 March 2024 2,195 1 View
Mostly bacteria living inside plant roots.
14 March 2024 4,602 0 View
The molecule is a copper complex and there are 3 such molecules in the CIF file
14 December 2023 4,999 2 View
I am optimizing crystal structure using quantum atk.In the output file it shows "Warning: The calculation did not converge to the requested tolerance!".Could anyone help me to solve the issue?I am...
04 November 2023 1,298 1 View
Hi, I am calculating the band structure of a double heterojunction with 116 ions using vasp. The geometric optimization and scf calculations are finished successfully. but when the band structure...
04 November 2023 8,310 0 View
Hi, I need to do electronic properties calculation on bigger systems like Bi2MoO6. DFTB in Material studio is found to be good for the process. But I dont know the fitting of parameters in...
02 November 2023 4,473 0 View
What is the correct way to calculate the BSSE correction for a molecule having 3 fragments? Please see the attached image for details. I know the values are pretty close but still I would love to...
25 October 2023 3,990 1 View
How do I calculate the stock solution to apply 100 uM MeJA for 1000 ml of plant nutrient medium ?
01 August 2024 723 0 View
I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...
23 July 2024 4,074 2 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
15 July 2024 5,898 0 View
methylene blue stock solution
19 June 2024 10,030 3 View
The emerging markets have suffered from a state of stagflation. I hope you know your expectations according to your experiences regarding the implications for the stock markets,,,, risks and...
19 June 2024 4,410 3 View
I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...
12 June 2024 6,087 0 View
After the addition of 2.5% ninhydrin solution (40% acetic acid/20% ethanol) to glycin samples for the STD curve, the highest concentrations quickly turn purple. However, after boiling for 20...
11 June 2024 5,592 1 View
I want to do Frap assay with ferrous sulphate heptahydrate (FeSO4.7H2O) as the standard. I have calculate the molarity which is 1 M or 1000 microMolar as the stock solution. How do I determine the...
11 June 2024 3,760 2 View
Hello, I recently started working with antibiotics and I do not have that much experience of making dilutions accurately. I want to ask if I suppose to add 200mg of antibiotic in 100ml of water...
08 June 2024 2,285 6 View
Hey everyone! I'm doing peptide synthesis on Rink amide resin and sometimes I need to add amino acids with secondary amines or beta amino acids. Normally I synthesize 35 to 40 mer peptides. As a...
21 May 2024 9,035 0 View