How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Which is the most efficient etching solution for nickel based alloys?

Which solution reveals the microstructure and features of nickel-based alloys?

02 March 2021 2,221 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View

Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

Problem in glucoamylase purification by gel filtration technique ?

31 December 2018 4,557 1 View

What is the relationship between starch content (%) and gelatinization?

I am currently doing a research on starch extraction using physicochemical cell disruption method (NaOH as solvent). The starch obtained upon disruption was decreasing from 10 mins to 30 mins at...

03 April 2018 6,719 3 View

What is the more relevant process for Blue -Iodine test (starch gelatinisation) for corn starch?

I couldn't find specific details about Iodine - Blue test used for determination of degree of gelatinisation of corn starch. Kindly give suggestions

07 August 2016 8,328 2 View

Does anyone have any idea about the following DSC curve?

I am wondering the appearance of first peak at 30 degree, my sample is a 10% starch gel, heating from 20 to 90 degree at 1degree/min. the second peak could be ascribed to gelatinization

02 March 2016 8,738 8 View

Has anyone experienced bovine PBMCs gelatinizing after 24 hours unstimulated?

I collected blood from a dairy cow and used a typical PBMC isolation protocol. Briefly, I diluted the sample 1:1 with PBS and centrifuged, removed the buffy coat and added it to 30ml PBS and then...

04 February 2016 671 2 View

Has anyone experienced bovine PBMCs gelatinizing after 24 hours unstimulated?

I collected blood from a dairy cow and used a typical PBMC isolation protocol. Briefly, I diluted the sample 1:1 with PBS and centrifuged, removed the buffy coat and added it to 30ml PBS and then...

01 February 2016 4,942 2 View

What are the examples of auxotrophic Saccharomyces cerevisiae yeast strains that can be used for ethanol production? What are their selective markers?

I am going to clone and express a glucoamylase gene in an auxotrophic Saccharomyces cerevisiae using YEp 352 which has a LEU2 and URA3 selective marker, in order to produce a one-step conversion...

31 December 2015 4,877 2 View

Why do some micro-organisms convert starch carbonate broth into gel ?

There is a m.o. that noticed conversion of a broth into gel. The medium is starch + carbonate .

03 April 2015 5,109 2 View

How should I proceed with LR Gold resin for small plant tissue embedding?

I would like to embed small pieces of plant tissue at RT in gelatin capsules with the use of LR Gold resin (Sigma), but unfortunately I have some problems with too fast polymerization. I added...

07 August 2014 151 3 View

Is there any method to assay glucoamylase using DNS or PNPG?

I want to know an easy way to assay glucoamylase activity in amylolytic yeast culture.

01 January 1970 6,905 1 View