Can I ask did any good platform or journals for learning the calcluation on the reference governors and command governor?

Recently I face some problems with the mathematical ( calculation) on the reference governor and command governor, so I need some help from you all to get which platform or journal can let me...

23 February 2021 7,746 2 View

Does anyone know the pH-differential method?

I use the water method to extract the anthocyanidin from Peristrophe bivalvis (L.) Merr leave, then dilute 20 times with 0.025M potassium chloride, pH1.0 and 0.4M sodium acetate buffer, pH4.5...

20 January 2021 8,305 4 View

Has anyone tried cross-linking cells with formaldehyde before co-immunoprecipitation?

Hi, I am currently trying to co-immunoprecipitate a protein but the interaction between that protein and its binding partner is not stable. Sometimes we able to detect its binding partner but...

29 November 2020 9,261 3 View

TOP/FOP-FLASH assay in HEK293T cells?

Has anyone tried to study Wnt signaling in HEK293T cells? I have been trying to measure the Wnt activity using TOP/FOP-FLASH assay but the readings that I got are rather similar in both TOP and...

28 August 2020 6,052 4 View

Does anyone have experience in docking metalloenzymes using dock 6.9?

14 August 2020 976 3 View

Serum starvation before transfection and cell doubling time?

Hi, everyone I have a simple question about timespan of serum starvation and transfection. Recently I work on U-2OS cells which have doubling time around 20 hours, and before I transfect U-2OS...

15 March 2020 3,293 4 View

Is it any way to do partition of edges not in same plane in Abaqus?

I am developing a model in Abaqus to simulate the cornea. I now want to partition the solid so as to set the middle part and outside part with different materials. However, I am having difficulty...

11 December 2019 7,645 1 View

What GLP-1 receptor antibody is good for IHC?

I'm trying to detect GLP1-R on mouse pancreas, I used proteintech 26196 1 AP at concentration 1:100, but it doesn't seem to work. Is there any other GLP-1R antibody that works well for IHC in...

25 November 2019 4,456 2 View

Where to get a journal to publish highly technical marketing research based on multivariate analysis or old-fashioned statistical methods?

where to get a journal to publish highly technical marketing research based on multivariate analysis or old-fashioned statistical methods?

30 May 2019 2,711 2 View

How to determine the composition of a complex sample containing organic compounds, amorphous and crystalline compound containing copper?

The sample is an intermediate product in the synthesis of CuO-doped TiO2 catalyst. Ethanoic acid is added and it reacts with copper nitrate to produce copper(II)acetate. A gel is prepared with sol...

15 January 2019 6,796 7 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

How can I make sure that BCTC doesn't fall out of solution/form DMSO crystals when treating cells?

I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...

14 February 2021 5,633 1 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

DMSO concentration in cell culture? Precipitating while PBS is added?

I am testing a compound on RAW 264.7. the compound is soluble in DMSO. but to ensure that final concentration of dmso is not more than 0.1%, we are first dissolving it in dmso and then diluting it...

11 February 2021 1,136 3 View

What are the main conditions suited for synthesizing a PVDF thin film by solvent casting method?

I have been trying to synthesize PVDF thin film by solvent casting method. I started by using 2:3 DMSO: acetone as solvents. The solution was stirred for 3hrs at 60 deg and was dried at 90 degrees...

09 February 2021 8,850 3 View

H2O2 scavenging activity problem??

When I do the test of plant against the free radicals of H2O2 the control had a value about 0.9126 The critical point is when I prepared the extract with DMSO the absorbance appear more than 2...

09 February 2021 6,430 2 View