I have 10X PBS pH 7.2. I want to use it to make 0.25m PBS, how can we calculate the M concentration 10x PBS.
We have been working on Banana streak virus. The PCR product which was sent for sequencing, after clonning, gives two different sequences from two different colonies. Similiarly, It was also...
04 May 2018 7,150 4 View
Can anyone tell me a few names of any Research journal which have High Impact Factor and quick in publication and also where the publication charges are free of cost or very low cost? I would like...
06 July 2016 5,410 6 View
Has there been any report on bacterial disease of cassia fistula? It has been reported to have possessed anti-microbial activity, but is there any report of its bacterial disease?
08 September 2015 3,057 2 View
Enzyme source was prepared with phosphate buffer, triton x and pvp. Now with dilution buffer as phosphate buffer for peroxidase and tris buffer for Pal. Can we calculate the enzyme activity? Will...
06 July 2014 4,222 3 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.
13 July 2024 7,462 1 View
I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.
08 July 2024 2,388 4 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!
26 June 2024 9,093 4 View
Hello, We are designing a protocol to determine the effect of certain compounds in the health of rats, but our problems have certain instability issues when subjected to certain pHs. We were...
19 June 2024 5,950 1 View
Hello, I am trying to purify a 6X-His tagged protein using Ni-NTA chromatography. However, I obtain very less yield & concentration; and considerable protein in load flow through. Any...
16 June 2024 5,876 5 View