How to prepare 0.25 M PBS from 10X PBS?

I have 10X PBS pH 7.2. I want to use it to make 0.25m PBS, how can we calculate the M concentration 10x PBS.

11 December 2018 305 0 View

One primer pair, one amplicon size but different genomic regions of same isolate. How can we explain to such findings?

We have been working on Banana streak virus. The PCR product which was sent for sequencing, after clonning, gives two different sequences from two different colonies. Similiarly, It was also...

04 May 2018 7,051 4 View

Any Journal with High Impact Factor and quick in publication and also where the publication charges are free?

06 July 2016 5,315 6 View

Can phenylalanine ammonia lyase assay and peroxidase be analyzed using the same enzyme substrate as the enzyme source?

Enzyme source was prepared with phosphate buffer, triton x and pvp. Now with dilution buffer as phosphate buffer for peroxidase and tris buffer for Pal. Can we calculate the enzyme activity? Will...

06 July 2014 4,122 3 View

How to perform EEG classification of Alzheimer and control groups?

I am doing a project on classification of EEG signals of Alzheimer's disease and control groups. Previously, I extracted the alpha power of the EEG signals and found out the strengths. I want a...

18 February 2021 8,502 2 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

13 February 2021 3,760 2 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Patients with chronic kidney disease could get COVID-19 vaccine?

04 February 2021 278 4 View

Is an Algal Microbial Fuel Cell a good research topic?

What do you think about studying the electricity production in an Algal Microbial Fuel Cell? Namely at the anode there is for example a solution with bacteria which degradate organic matter into...

27 January 2021 5,681 5 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

How can you accurately induce calcium overload and measure membrane potential in cultured Tg2576 neuronal cells using whole cell patch clamp method?

Hi there, I'm currently working on a project where i need to design an experiment but as i have little to no experience with this level of experimental design and no face to face help i am really...

22 January 2021 6,728 3 View

Where can I get a Music therapy database for Alzheimer's Disease?

I am working on the effect of music therapy on persons with Alzheimer's Disease. I require EEG signals of healthy individuals and people with Alzheimer's Disease/Dementia that have undergone music...

22 January 2021 8,613 5 View