I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

SDS-Gel stacking gel polymerization issues?

Can anyone provide any insight as to why my Tris/Gly SDS-Gels are polymerizing slowly? This especially applies to my stacking gel (image attached) both 4% and 6%. The stacking gel wells polymerize...

24 January 2021 3,791 4 View

Will rat colon cancer cells (DHD/K12) derived from BDIX rats grow in Sprague Dawley ?

we plan to induce colon cancer by injection. Cell lines derive from BDIX rats, but our experimental setting would favour SD rats. any experience?

23 January 2021 1,437 2 View

I am interested in sequencing small RNAs in bacteria but enriching for small, weakly expressed transcripts seems to be a challenge. Any ideas?

Assays for bacterial small RNA sequencing

06 January 2021 4,549 2 View

What is the white layer on my fermented natto bean paste?

Dear all, Good day! Recently i tried to ferment my blended soya bean powder mixed with Natto bacteria culture in a Natto maker (containing some water) at around 45 degree celsius overnight....

23 November 2020 7,105 20 View

How do I extract mice brains for multiple experiments?

I am looking to complete western blot experiments and IHC experiments on the same cohort of mice. Is it possible to Harvest the mouse and use the right hemisphere for IHC and the left for western...

08 September 2020 5,436 3 View

Best way to add streptavidin onto flat surface?

I will be adding streptavidin onto the surface of a graphene coated glass slide; thereafter, I will be analyzing with a MALDI-TOF. I was wondering the best way to go about this? I was thinking of...

07 September 2020 5,902 4 View

Why the need to have CIE formula when color difference can be measured with RGB values?

Dear all, Recently i have been trying to measure colour difference between my samples. I am a little confused as to why i cannot just use the basic colours - i.e., red, green and blue to measure...

02 August 2020 7,375 2 View

Does anyone know of an Antibody against against Mouse Microglia trf2 that is good for Immunofluorescence and Westernblot?

Hi, I am studying the knockdown of Trf2 protein in Murine microglia cells invitro and wish to demonstrate this knockdown via immunofluorescence and Western blot. A dependable Trf2 antibody and...

02 June 2020 8,682 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

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25 February 2021 5,713 1 View