Dear SurjitBhai: You are posing any question on this forum. Why don't you try to be specific in your questions. To give you a broad-viewanswer please read the basics of bioinformatics as given in Rastogi's book.

A simple procedure would be digest the whole genome partially with a single restriction enzyme and create a DNA library of 2 Kb size. If genome is too large, maintain an mRNA library. Check all the members of library for their ability to grow on the required antibiotic containing media.

Stepwise procedure (For unknown antibiotics):

1. Grow the marine Actinobacteria and collect the cell free supernatant (CFS).

2. Create wells using well-borer on a NB agar plates (Or appropriate media) and perform spread plating of common pathogens (A few bacteria and molds etc.).

3. Now fell the wells with different dilutions of the CFS and use commercial antibiotics as positive control and uninoculated broth as negative control.

4. Incubate for appropriate time and check for zone of inhibition.

5. If you see any activity, then take that CFS and go for purification (Antibiotics can be peptides also. Therefore, systematically purify for different types of antibiotics).

6. Again perform the zone of inhibition assay with any step of the purification.

7. For antimicrobial peptides, perform SDS-PAGE and extract different bands from the gel and again perform the zone of inhibition assay.

It will take a lot of time to perform these experiments and get a novel antibiotics. All the best!

Thnx a lot