Surjit Singh

7 Questions 17 Answers 0 Followers

Questions related from Surjit Singh

How to verify the RDP classifier results and taxonomic assignment at species level in BLAST?

I am analysing 454 pyrosequencing data for the identification of bacterial diversity by 16S rRNA gene sequencing. I have used RDP classifier for taxonomic assignment and now I want to cross verify...

17 March 2016 7,988 3 View

How to collect root exudates from rice plants growing in soil and hydroponics?

I am growing rice plant (IR64) in both soil and hydroponics and i want to collect the root exudates from soil and hydroponics. As it has been said for a long time that rice is a tough plant to...

26 February 2016 5,086 5 View

Can anyone provide me the standard protocol of isolation of total DNA, RNA and Protein content from rhizospheric soil of rice?

I want to quantify the microbial diversity in the rhizospheric soil of paddy field. kindly provide me the standard protocol with references. Thank you

17 September 2015 6,970 4 View

How to draw an RAPD dendogram?

I want to draw a dendogram to compare the genomic differences between two bacterial genomes.

22 February 2013 1,009 11 View

How can I purify bioactive compounds - especially unknown antibiotics from Nocardiopsis?

Solvent extraction was done by ethyl acetate.

17 February 2013 5,307 2 View

How to separate unknown antibiotics by using thin layer chromatography?

antibiotics from Marine actinobacteria

17 January 2013 2,494 1 View

How to predict and isolate gene which produces unknown antibiotics?

Antibiotics from marine actinobacteria

17 January 2013 3,407 4 View