Siva Ramakrishna Uppalapati

3 Questions 25 Answers 0 Followers

Questions related from Siva Ramakrishna Uppalapati

Transfer RNA acylation levels?

Hi all, I m trying to estimate relative levels of bacterial tRNA (acylated and deacylated forms) by northern blot. Recently I realized I should use acid-Urea PAGE for differentiating these two...

17 August 2021 7,760 0 View

CRISPR Cas9 editing for small RNA deletion ?

Hi, Does anyone have experience in RNA editing with CRISPR Cas9 method? Does this tool have application limited only to gene editing or I am wondering whether intergenic regions that code for...

20 April 2018 8,903 3 View

Is it necessary to quantify RNA concentration before cDNA preparation from bacteria?

Is it necessary to quantify RNA every time before cDNA conversion or can we just quantify cDNA after reverse transcription? How much difference does it make in relative quantification experiments?

19 November 2012 2,675 7 View