02 March 2022 5 9K Report

I wish to purify the outer membrane proteins of E. coli. The methods available involve the ultracentrifugation as mentioned by Choi-Kim et al. (1991). I wish not to use ultracentrifugation. Can anyone suggest any other method involving precipitation of OMPs.

Thanking you in advance.

Choi-Kim, K., Maheswaran, S. K., Felice, L. J., & Molitor, T. W. (1991). Relationship between the iron regulated outer membrane proteins and the outer membrane proteins of in vivo grown Pasteurella multocida. Veterinary Microbiology, 28(1), 75–92. https://doi.org/10.1016/0378-1135(91)90100-T

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