17 Questions 20 Answers 0 Followers
Questions related from Shantanu Tamuly
For any new vaccine antigen, it is inoculated to target species and the blood is collected to assess the antibody titre. And in a particular time, the subject is challenged with the live organism....
28 May 2023 4,809 3 View
Can we use the descicator as anaerobic chamber for incubation of Clostridium perfringens.
28 May 2023 4,190 0 View
The method described by A.M. SOLER-RODRIGUEZ and J.M. ARMAS L. utilizes the Candida species for determining phagocytic activity of PMNL. Is there any other simple and cheap method that uses any...
18 March 2022 1,157 11 View
I wish to purify the outer membrane proteins of E. coli. The methods available involve the ultracentrifugation as mentioned by Choi-Kim et al. (1991). I wish not to use ultracentrifugation. Can...
02 March 2022 9,061 5 View
I am trying to make Sabouraud Dextrose Agar for growing Candida albicans. I am using the following ingredients: Peptone 10 grams Dextrose: 40 grams Agar: 15 grams Distilled water added up to 1000...
15 February 2022 3,861 6 View
Hi all, I am using the following code to obtain the bar plot uisng the R package Ggpubr. I am required to add texture into the bar plots instead of color. I could not find any option for adding...
30 July 2020 1,139 3 View
I ran the response surface modeling using central composite design. And I got this output. Can I take this model? The model is having lack of fit p value more than 0.05. But other parameters are...
24 February 2020 6,972 4 View
What is the reason that most of the researcher uses mice for evaluating immune response of vaccines. Why nobody uses rat for the same purpose?
18 November 2019 1,380 3 View
Hi All, I performed RSM in my data with two factors pH and cond. and my response variable is DBC. After conducting the RSM analysis in RSM package of R I got the one stationary points in original...
18 March 2019 1,816 3 View
Could some advise me from where can I obtain P22 phage in India?
14 March 2019 3,493 0 View
Hi, I am trying to digest my insert of 8.5 kb long with NdeI and XhoI (both obtained from NEB) but the insert gets destroyed after the digestion. I do not see anything in agarose gel. Kindly...
21 February 2019 8,908 8 View
I am doing PCR amplification of 8.5 kb DNA fragment. I have observed a wierd result. When I use a master mix of 50 micL, I get the amplification but when I use 100 micL reaction it does not give...
11 February 2019 9,769 4 View
Hi, I am using pFLAG-CTC vector for cloning a DNA fragment of 8.5 kb. I have been struggling but failed to get a proper clone. Last time I got a clone but it carried an insert of less than 8.5...
30 January 2019 2,748 1 View
I am trying to clone an 8 KB fragment to pFLAG-CTC vector. The vector map of the pFLAG-CTC vector is attached herewith. The issue I am facing is that after ligation reaction, and transformation...
12 January 2019 3,265 7 View
I am doing indirect ELISA for estimation of specific IgM antibodies in poultry chicken. I would like to know what should be dilution of anti-chicken IgM HRP conjugate that should I take for...
02 June 2018 6,800 3 View
In my experiment, I have four groups namely A, B, and C. I have made the observations in all three groups in 4 different days namely 7th , 14th, 21st and 28th day. Now, I want to perform a...
09 May 2018 9,670 10 View
I am doing the Repeat Measure ANOVA using a below mentioned model in R. But I am not understanding how to perform pair-wise multiple comparison. I have added one hypothetical data in the file...
12 February 2017 9,785 5 View
