14 Questions 18 Answers 0 Followers
Questions related from Shantanu Tamuly
For any new vaccine antigen, it is inoculated to target species and the blood is collected to assess the antibody titre. And in a particular time, the subject is challenged with the live organism....
28 May 2023 4,722 3 View
Can we use the descicator as anaerobic chamber for incubation of Clostridium perfringens.
28 May 2023 4,113 0 View
I wish to purify the outer membrane proteins of E. coli. The methods available involve the ultracentrifugation as mentioned by Choi-Kim et al. (1991). I wish not to use ultracentrifugation. Can...
02 March 2022 8,993 5 View
Hi all, I am using the following code to obtain the bar plot uisng the R package Ggpubr. I am required to add texture into the bar plots instead of color. I could not find any option for adding...
30 July 2020 1,031 3 View
I ran the response surface modeling using central composite design. And I got this output. Can I take this model? The model is having lack of fit p value more than 0.05. But other parameters are...
24 February 2020 6,871 4 View
What is the reason that most of the researcher uses mice for evaluating immune response of vaccines. Why nobody uses rat for the same purpose?
18 November 2019 1,296 3 View
Hi All, I performed RSM in my data with two factors pH and cond. and my response variable is DBC. After conducting the RSM analysis in RSM package of R I got the one stationary points in original...
18 March 2019 1,754 3 View
Hi, I am trying to digest my insert of 8.5 kb long with NdeI and XhoI (both obtained from NEB) but the insert gets destroyed after the digestion. I do not see anything in agarose gel. Kindly...
21 February 2019 8,852 8 View
I am doing PCR amplification of 8.5 kb DNA fragment. I have observed a wierd result. When I use a master mix of 50 micL, I get the amplification but when I use 100 micL reaction it does not give...
11 February 2019 9,692 4 View
Hi, I am using pFLAG-CTC vector for cloning a DNA fragment of 8.5 kb. I have been struggling but failed to get a proper clone. Last time I got a clone but it carried an insert of less than 8.5...
30 January 2019 2,684 1 View
I am trying to clone an 8 KB fragment to pFLAG-CTC vector. The vector map of the pFLAG-CTC vector is attached herewith. The issue I am facing is that after ligation reaction, and transformation...
12 January 2019 3,178 7 View
I am doing indirect ELISA for estimation of specific IgM antibodies in poultry chicken. I would like to know what should be dilution of anti-chicken IgM HRP conjugate that should I take for...
02 June 2018 6,744 3 View
In my experiment, I have four groups namely A, B, and C. I have made the observations in all three groups in 4 different days namely 7th , 14th, 21st and 28th day. Now, I want to perform a...
09 May 2018 9,606 10 View
I am doing the Repeat Measure ANOVA using a below mentioned model in R. But I am not understanding how to perform pair-wise multiple comparison. I have added one hypothetical data in the file...
12 February 2017 9,718 5 View
