I would like to know please what is the best way to plot curve to calculate IC50.
for 3 drugs, FRET kinase binding assay was performed through a service provided by a company, as those 3 drugs are supposed to inhibit certain kinase. The results were in the format of inhibition % under different or multiple concentrations (100uM, 50uM,10uM, 5uM, 1uM, 0.1uM)
what I need to know,please, is it better to plot the inhibition % on y-axis, against what? the multiple concentrations or the log(concentrations).???
The point is that i need to calculate IC50 and Hill slope ? so which way is supposed to be plotted for each of the IC50 and the Hill slope.??
Thanks a lot
Because the concentrations tested cover 4 orders of magnitude, you should plot them on the x-axis on a log scale. The percent inhibitions go on the y-axis. Use nonlinear regression to the Hill equation to calculate the IC50
% inhibition = 100[I]n/(IC50 + [I]n) where [I] is the inhibitor concentration and n is the Hill slope.
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