Hi all,
We are going to house mice for a double-ko investigation. Since this is our first time housing mice, we have no knowledge on how we should optimize breeding.
So here's the info:
One gene is on Chr-X, the other on Chr-11. Both males and females homozygous double-ko should be infertile. The simple ko are supposed to be ok. We are mostly interested in ovaries of the double-ko females, but we'll also check on both simple-ko and wt as controls.
So what strains should we ask to start the colony ? We also want to cryopreserve embryo in the first few weeks.
The collaborator who is going to send the mice proposed this:
Males: Gene1(-/0), Gene2(-/+)
Females: Gene1(-/+), Gene2(-/+)
Do you think that is an appropriate start ?
In my opinion, If you cross male and female of given pair:
Theoreticallly, Your chances on getting one male double KO and one female double KO in 16 pups if all are alive (you will keep on breeding this pair till you get enough numbers), The details lies here:
(1) 1-/- 2-/- Female Double KO
(2) 1-/0 2-/+ Male
(3) 1-/0 2-/- Male Double KO
(4) 1-/- 2-/+ Female
(5) 1-/- 2-/+ Female
(6) 1-/0 2+/+ Male
(7) 1-/0 2-/+ Male
(8) 1-/- 2+/+ Female
(9) 1-/+ 2-/- Female
(10) 1+/0 2-/+ Male
(11) 1+/0 2-/- Male
(12) 1+/- 2+/- Female
(13) 1-/+ 2-/+ Female
(14) 1+/0 2+/+ Male
(15) 1+/0 2-/+ Male
(16) 1-/+ 2+/+ Female
Once you get a pair of double KOs, and if they are fertile (depending on your genes not affecting male and female fertility), You will be lucky that you can breed among them and get all pups should be double KOs.
Alternatively,
for next breedings, you can may include female mouse number 9 and male number 11 for better results if they are fertile
Hope that helps, let us see if someone comes up with better strategy,
Subhash
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