I am constructing a phylogenetic tree using align sequences of 4 mt-genes [2 coding, 2 noncoding]. Total nucleotide length extends up to about 2500bp. I am stuck in one point, therefore I want to get into an exact idea on how to determine the exact numbers for following;
*Number of generations
*Sample frequency
*Diagnostic frequency
*Number of parallel runs
*Number of heat chains
If someone could help me with this I would be much grateful.
Thank you..