I am constructing a phylogenetic tree using align sequences of 4 mt-genes [2 coding, 2 noncoding]. Total nucleotide length extends up to about 2500bp. I am stuck in one point, therefore I want to get into an exact idea on how to determine the exact numbers for following;

*Number of generations

*Sample frequency

*Diagnostic frequency

*Number of parallel runs

*Number of heat chains

If someone could help me with this I would be much grateful.

Thank you..

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