Can aminopeptidase be used with proteinase k in plasma protein digestion? I hope to digest the protein before DNA extration. Anyone know that? Thanks a lot.
Can diamond be grown using molecular beam epitaxy?
22 July 2024 9,755 2 View
In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...
15 July 2024 1,929 2 View
Yuen P.H. 1965. Some studies on the taxonomy and development of some Rhabdisoid and Cosmocercoid nematodes from Malayan amphibians. Zoologischer Anzeiger, 174, 275–298. Thank you very much for...
07 May 2024 9,971 0 View
(1) SHARPILO, V. P. 1961. New nematode Spauligodon saxicolae nov. sp. Parasite of the scaly lizard Lacerta saxicola Evers. Trudy Ukrainskoe Respublikanskoe Nauchnoe Obshchestvo Parazitolohov 1:...
02 May 2024 4,116 0 View
Sharpilo, C. P. (1976). Parasitic worms of the reptilian fauna of the USSR: Systematics, chorology, biology. Moscow: Naukova Dumka, 287 pp (In Russian). Thank you for all your kind helps!
02 May 2024 2,013 4 View
Hello everyone, I am currentlyseeking scanned or digital copies of the following books: Angelika Linke (1985): Gespräche im Fernsehen: eine diskursanalytische Untersuchung. Bern: Peter...
20 April 2024 9,178 0 View
What's the role of Live Stream influencer Marketing on Consumer Brand Engagement and Brand Preference?
16 April 2024 229 1 View
I need to add NH4NO3 and KH2PO4 as nutrients for seagrass growth to the artificial water which is made from sea salt, but I only know the previous data and don’t know how to calculate how many...
25 February 2024 778 7 View
Basically most studies of "structural equation modelling + time-series data" focus on longitudinal datasets (e.g., (residual) dynamic SEM). However, for a questionnaire survey or measure at a...
21 November 2023 7,979 0 View
Could I obtain a compact film through the atomic layer deposition method? If yes, why as-deposited SnO2 film with a thickness of 17 nm fail to protect the underneath layer film of my device? Could...
05 November 2023 8,153 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View