How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to use the results of Co-IP for protein quantitative difference calculation?

I did a Co-IP MS with two samples and got the LFQ data from MaxQuant, and I wanted to normalize it by an internal reference protein. After I divide the LFQ of the other protein by the LFQ of the...

18 January 2021 5,419 4 View

Does anybody have the recipe for making a home made R electroporation buffer for Neon transfection systems?

I tried the recipes below in two cells lines: 1.DPBS 2.DPBS+MgCl2(1mM) 3.DPBS+Sucrose(250mM) 4.DPBS+MgCl2(1mM)+Sucrose(250mM) 5.R Buffer (Neon) the results showed that R buffer is the best one in...

06 January 2021 6,776 1 View

How to Draw Crystal Structure from powder XRD?

Hi, I would appreciate if anyone can recommend on the process of drawing crystal structure from observed powder xrd diffractogram and if there is any suggested software to work on.

05 January 2021 3,517 3 View

Why mass flowrates of atoms at the inlet and outlet of a reactor are not conserved (CFD with chemical reactions, species model - eddy dissipation)?

Hi, guys. Thanks for reading this question. I am modeling a reactor (steady, RANS). CH3OH and O2 go into the reactor via inlet (a jet). After reactions, species leave the computational domain...

08 December 2020 7,362 13 View

Is the pH value will affect the calculated overpotential in OER process by DFT?

27 November 2020 1,456 2 View

How to transform this implicit FDTD iteration equation into the matrix equation?

11 October 2020 9,042 1 View

What is the best way to evaporate a large volume of aqueous solution quickly?

Let's say I have 100 mL of an aqueous solution and I wish to reduce the volume to 5 - 10 mL. What's my best option? - Rotavap is ruled out because we do not have a vacuum pump that can create...

09 September 2020 2,534 8 View

Bugs in mouse genotyping?

Hi, I am genotyping newly constructed trangenic mice knocking down target gene. I have got F1 generation from F0. And DNA examination indicates that the target DNA fragment has been excised. The...

27 April 2020 5,450 3 View

Will there be a large temperature gradient in the cross-section of a steel member under fire?

I have been working on the behaviors of steel structures at evaluated temperatures. We all know that heat can be easily transferred through steel. So, in real cases, will there be a large...

19 March 2020 1,748 5 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View