How to perform in vitro beta-oxidation of a xenobiotic?

More Arianna Giorgetti's questions See All

Veeco Nanoscope IIIa Troubleshooting -- How to Fix Not Turning On?

My school recently acquired a Veeco Multimode and Nanoscope IIIa. While our software and computer is set up and working, the actual Nanoscope is not powering on. We currently have it wired up, and...

19 June 2024 592 0 View

Hydrogen: are you a NIMBY or a YIMBY?

Hydrogen is seen a promising decarbonisation route for many energy-related sectors. However, like for other energy innovations, the NIMBY syndrome may turn it into something socially...

30 May 2024 7,063 0 View

Application of The Generalized Method of Moments?

The professor gave us an exercise but for me it's not totally clear. I have a distribution function which depend on a parameter X that I need to estimate. The function describes the distribution...

03 January 2024 603 0 View

How to remove Bisphenol A (BPA) from glass bekers?

I exposed some organisms to BPA at low concentrations (10-100 µg/L) for 1 month and now I need to get the bekers clean. Does anybody know if and how much BPA sticks to glass and which is the best...

27 July 2023 3,186 0 View

Is anyone having trouble accessing the IBM functional genomics platform?

It says they are updating the developer software, but has been like this for a week. Any updates on this? I would like to gather data from this platform.

30 May 2023 3,810 1 View

Is it possible to directly count Spirulina cells in a counting chamber?

I am looking for a direct method to quantify Spirulina biomass. What kind of slide should I use to count cells or filaments?

29 January 2023 9,079 0 View

Is it possible to use the DIF method to test the expected item response by comparing two different measures in the same population?

Hi everyone, I have a question: is it possible to use the DIF method to test the expected item response by comparing two different measures in the same population? Sometimes, DIF is tested...

09 May 2022 9,014 0 View

How to decrease DNA shearing with Qiagen QIAamp DNA micro kit?

I am working with CD4+ cells isolated from 15-20 year old HIV + PBMCs in DMSO stored in LN2 freezers with minimal freezing and thawing. I'm running a digital droplet PCR on the extracted CD4+ DNA...

01 December 2021 214 0 View

Scientific illustrations software/course?

Hi everyone, I'm a med-chem PhD student and I would like to improve my skills in creating scientific illustrations for articles and conferences. Can you please suggest some software (better if...

20 September 2021 4,314 2 View

Which is the best review software for conducting a Scoping Review?

Hi, I have a question: between the software Covidence, JBI SUMARI and Rayyan, which is the most appropriate and functional for conducting a scoping review? I will appreciate any...

18 April 2021 6,543 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Help on understanding the implementation of Mori Tanaka method on MATLAB?

I am new to Micromechanics and having similar problem with understanding the implementation of the formula's. I would appreciate if anyone can guide me on how to go about getting a scalar value...

30 July 2024 969 0 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How can parents ensure their drinking water is free from harmful contaminants?

27 July 2024 8,722 6 View

How to select relevant metabolites from multiple annotations ?

I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...

24 July 2024 5,789 0 View