Western blot in brown adipose tissue?

Good morning, I am a PhD student trying to get my head around brown adipose tissue western blot. So far I have successfully isolated proteins with high molecular weight such as PGC1a but I keep...

01 August 2020 8,764 3 View

How big are the neurons in zebrafish? Are there any particular stains useful for label just neurons?

12 September 2017 1,696 3 View

Can anyone provide advice on how to purify an S-tagged protein?

I am having trouble purifying my S-tagged protein. All protocols I have read call for batch mode, but say that column mode is also doable. I am using a column with S-protein Agarose from Novagen,...

01 January 2013 5,034 6 View

Does anyone have any suggestions on how to prevent proteolysis during purification of a His-tagged protein?

I am currently in the process of purifying a His-tagged protein. I use a Nickel column and then an ion exchange column to further get rid of other proteins. I have been pretty successful, but I am...

12 August 2012 3,985 24 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

No title

02 March 2021 324 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View