How do I detect primer dimers in an electrophoresis gel? and how to avoid them in a PCR reaction?

Hello! I am investigating plant-pollinator interactions by means of metabarcoding of pollen loads. The approach we want to use is dual-indexing in a two step PCR process. We are using four...

04 May 2020 7,455 6 View

Hello, I want to know if someone ever did a western blot with an expired mops running buffer with precast gels.

Hello, I want to know if someone ever did a western blot with an expired mops running buffer with precast gels. I used it for my last gel and the ladder was faint on the gel and obviously on the...

19 July 2017 1,948 7 View

Can anyone help me to find free articles about syllable structure and sonority in aphasia?

Thanks!

10 January 2015 4,945 3 View

How to compare sample values from 2 different western blots?

How can comparison between phosphorylated and total protein amounts be made without stripping and re-probing the same gel but using two gels, one for phosphorylated and the other for total...

19 September 2013 7,226 3 View

Graphene coatings

Hello! I have just been assigned a new job task - to help with the product development of the company I work for. The aim is to develop a graphene coating (amongst other functional coatings) but I...

30 June 2013 1,680 2 View

Please participate in my study! Adverse childhood experiences and adult functioning - EDIT/UPDATE

THANKS TO EVERYONE WHO PARTICIPATED. THE SURVEY IS NOW CLOSED.

01 January 1970 1,443 4 View

Would you like about neuro-scientific research of violent video game and media effects?

01 January 1970 5,916 1 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View