Good day, I need to perform a viral load analysis to quantify the number of viral particles/mL found in my samples. The virus that I will be working is with the Human immunodeficiency virus type I. I was wondering, how do you perform a viral load analysis? I have my samples per mL frozen in liquid nitrogen, where do I go from there for quantification?
Hi, carefully read through the manual of the kits you will use and ensure you follow the instructions carefully.
Just to clarify, do you mean virus preparations when you say samples? I used to measure lentivirus titers and this involved infecting HEK293 cells with GFP expressing virus preparations and then enumerating the frequencies of infected cells using flow cytometry. You could then back calculate the viral titer.
Well, I have to infect peripheral blood mononuclear cells with HIV-1 particles for 9 days for viral propagation and then save the supernatant into separate cryovial tubes to create a bank of virions. I would need to need measure how many particles are present per mL. Any technique for such task?
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Condensed Matter Physics
- Soft Matter
- Liquids