Wat is the half-life of luciferase protein in mouse tissue when delivered using mRNA luciferase?

Hello, I I am validating a system to deliver mRNA using nanoparticles to a mouse model. I am initially using mRNA for firefly luciferase to be able to do live imaging in the mouse. After that, I...

17 February 2021 2,536 3 View

DoE: Possible reasons for having a lot of significant effects? Is it as complex or went something wrong?

I did a product development using Design Expert for planning and evaluation. There is a response with a lot of significant effects shown in the Half Normal Plot. By testing factors A, B, C, D I...

06 December 2020 5,128 3 View

How to know if 87Sr/86Sr is reliable for Sr isotope chemostratigraphy in marbles?

I am currently looking for parameters to know if 87Sr/86Sr data is reliable for chemostratigrahy in marbles, and/or parameters to know if those marbles have had some post-depositional...

30 November 2020 9,032 8 View

Controlling for a variable that is an outcome of the dependent variable: what is the interpretation of the coefficient of the regressor?

I am estimating a dependent variable as a function of a regressor using Ordinary Least Squares regression. If I add a control variable that is (partly) an outcome of my dependent variable, how do...

06 October 2020 3,224 1 View

Which EEG source analysis plugin for Matlab do you recommend?

Good morning, I have the EEG recordings of 65 subjects and I would like to reconstruct the source of the signal in the gamma frequency range. Unfortunately, I don't have an individual MRI or...

25 August 2020 10,021 2 View

How can I study the clusterization Vglut1 puncta in cultured cells ? Do you know exactly the dimension of one single Vglut puncta?

Hi guys, I ave to study the Vglut1 puncta density/clusterization in cultured cells and see any differences between 2 different conditions. I noticed that in treatment B Vglut puncta appear...

29 March 2020 6,120 1 View

Botany - Betula pubescens old drawing?

Does any of you have a vintage botanical print of Betula pubescens (alba) subsp. celtiberica? Thanks a lot!!!

27 February 2020 6,049 5 View

Why do I obtain wavy bands on acrylammide precast gel in western blot experiments?

Hello! I recently tested a new precast gradiented gel for my Western Blot experiments and I obtained wavy bands both for proteins and for molecular weight marker. I run in tris-glycine-sds running...

28 July 2019 2,303 6 View

Tris HCl 1.5M pH 8.8, Tris HCl 1 M pH 6.8, how to pH?

I am experiencing many problems in obtaining the correct pH of Tris-HCl solutions for acrylammide gels (pH8.8 and 6.8) due to the dependence of the pH on temperature. I prepared and brought the...

18 June 2019 7,663 9 View

Does anyone has any references for scholarly articles on teacher professional development and teacher identity?

My field research was to study the interactions of teacher leaders in a teacher leader PLC in a primary school. I am curious as to any findings you'd be willing to share and any current scholarly...

30 December 2018 8,201 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View