How to over-express a selected circular RNA?

07 July 2018 1 8K Report

Dear All

Do you have experience in methods for the over-expression of selected circular RNAs in human or mouse cells?... If yes... which strategy did you use?... which vectors and promoters?...

Thanks in advance. Best

Paco

Ajit kumar Roy

A great diversity of circular RNAs (circRNAs) can be generated from a single genomic locus. These circRNAs are created through a non-canonical splicing process known as ‘backsplicing’ in which a downstream splice donor is joined to an upstream splice acceptor. Such circRNAs can consist of one or more exons and can even contain unspliced intronic sequences. Colored bars, exons; black lines, introns.

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