I hope that following information will be relevant.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3916676/

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004516/

HI Hasnaa,

Gregory's links are indeed much appropriate. PMP detection iand more generally EV(extra-cellular vesicles) analysis and counting is actually a hot topic. For your and general information, 3 major scientific societies (i.e. ISAC, ISEV and ISTH) have recently expressed their willing to collaborate for improving the field together. This was announced at CYTO 2015 (june 2015 in Glasgow, UK) and will surely be again actively discussed and setup at ISEV 2016 (Rotterdam, NL, 4-8 May 2016), ISTH SSC (Montpellier, F, May 25-28, 2016) and CYTO 2016 (Seattle, June 2016). 

My suggestion is to look for two special issues on Microvesicles just coming out i) in TRASCI (Transfusion & Apheresis Science) as well as ii) In Press in Cytometry A,(papers also available as doi.)

I point mines to you (also listed in my RG page) to give the website links to the journals and help you find the many very interesting contributions around, in each special issue. If you do not find focused enough information, do not hesitate to come back again and re-focus your question. .

Best regards. Philippe

Journal: Cytometry Part A

Article title: "Standardized counting of circulating Platelet Microparticles using currently available flow cytometers and scatter-based triggering: Forward or Side Scatter?"       DOI: 10.1002/cyto.a.22685

Article title: Tips and tricks for flow cytometry-based analysis and counting of microparticles.

Article reference: TRASCI1912

Journal title: Transfusion and Apheresis Science

 First author: Dr. Philippe Poncelet

Accepted manuscript (unedited version) available online: 27-OCT-2015

DOI information: 10.1016/j.transci.2015.10.008

we are here in cairo university working on a protocol to measure RBCs microparticles. does anyone have recommendations on best way to measure and to avoid contamination