I would like to know if there is a method that allows us to obtain PK "proteinase k" from natural biological substances without going through the trade and that will allow us to perform a DNA extraction and obtain a " medusa"
Which methods or bioinformatic tools can be used to identify new SNPs on an exon or a region of a promoter of a gene of interest in silico
07 August 2017 7,510 2 View
If, for example, there are two genes encoding two different proteins on the same chromosome that have an interest in a plurigenic pathology, what bioinformatic tool or method can be used to show...
07 August 2017 2,619 0 View
I used ChIP-Seq data to map DNA binding sites of a protein of interest at the genomic scale, is it mandatory to control the quality of sequencing even if there are referential data in NCBI?To...
06 July 2017 8,067 13 View
The chi-square test is a continuous probability law and at the same time is a nonparametric test which compares qualitative data with the use of small samples in the sub-groups but not in the...
06 July 2017 4,354 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...
09 August 2024 5,444 7 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View