Protein should be in its native form after extraction from pellet.
I think affinity column chromatography would help you in this.
Hello all, I've compiled a udf which reads velocity data with corresponding time stamp from a .csv file using DEFINE_ON_DEMAND macro. Then, I use DEFINE_PROFILE to define an inlet velocity profile...
14 June 2024 6,480 0 View
I'm currently studying the treatment of sewage wastewater and am interested in understanding the impact of autoclaving on the concentration of heavy metals. Specifically, I would like to know if...
10 June 2024 2,579 4 View
I have installed a linux system for the same purpose and downloaded the VIC-4.2.d model. While looking for any tutorial series and manual for use and operation of VIC is not found. I would be very...
29 April 2024 9,097 1 View
no
13 April 2024 1,479 3 View
I used TBAPF6 electrolyte for CV study but it generated anion of my compound. Please suggest me which electrolyte would be suitable for cyclic voltametric study of mild acidic organic compounds?
11 March 2024 1,116 4 View
Can anyone help me calculate Cremer & Pople puckering parameters from a PDB or XYZ (or Cif) file using PLATON software, online, or any other simple method? (free downloadable windows supported...
02 March 2024 8,945 1 View
Tried to use PVDF with NMP and DMF but slurry couldn't be prepared.
15 January 2024 5,790 0 View
I am been using VEROTMPRSS2 cells and incubating cells with 200ul of virus dilution for one hour. Cell layers are coming off after 30 min incubation. What might be the best solution to keep cell...
07 November 2023 9,802 2 View
I calculated anisotropic hyperfine parameter using gaussian 9 and got values as follows: For example: Atoms MegaHertz C(13) Baa -6.995 Bbb...
05 November 2023 8,357 0 View
I want to know about the best method for RNA sequencing- mRNA capture or Ribo depletion.
10 October 2023 8,697 1 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View