How to obtain desired protein from a pellet, as the protein of interest is coming in the pellet?

10 July 2012 1 2K Report

Protein should be in its native form after extraction from pellet.

Badges
Science method

More Poonam Roshan's questions See All

What is causing the raw fluorescence to be negative?

Hi everyone, I would really appreciate it if someone can tell me when and why a raw fluorescence starts below zero. In the picture attached below the blue colour curve starts from a negative...

09 February 2021 4,642 3 View

Why there is need to calculate the chlorophyll content and chlorophyll index. What protocol should I used for calculating chlorphyll in mustard?

I want to know the best and effective method/ protocol to calculate the chlorophyll from mustard plants. Please suggest some relevant and the methods or protocols from which I can easily calculate...

05 January 2021 9,882 3 View

Which staining method is best for Archaea?

Generally to differentiate between bacteria we use gram staining technique, broadly based on the composition of their cell wall. However archaebacteria have different cell wall composition, which...

14 December 2020 9,703 5 View

Can a subclinical case of a rare syndrome be published as a Clinical diagnosis?

This case is important from a clinical point of view. Karyotype was normal. Advanced genetic tests were not conducted due to unavailability of such tests in the state as well as financial concerns.

07 October 2020 398 3 View

How can I simulate a droplet motion on a digital microfluidic chip using COMSOL Multiphysics??

Hi, I'm trying to simulate a DMF (digital microfluidic) chip in COMSOL Multiphysics 5.5. I used a 2D model and selected two phase flow, level set and electrostatic modules to simulate a droplet on...

26 September 2020 1,392 6 View

How can variables be clustered in few dimensions?

30 participants have given 50 adjectives (maximum 3 adjectives from each participants) for a phenomena. Now I want to to club/cluster these 50 different adjectives in fewer (say 8-10) adjectives....

24 September 2020 9,889 5 View

How to recycle Sodium Iodide?

Hi, I am using sodium iodide to separate density carbon fractions of my samples. I want to recyle it for further uses. While searching I only get the process to recycle sodium polytungstate (at...

20 September 2020 9,556 2 View

Statistical Analysis test?

Dear researchers, How to know which and how many statistical test to apply for the collected data while doing research as we have lots of parametric and non parametric test available especially...

09 September 2020 688 3 View

SPSS extension plugin?

Dear researchers, I have downloaded BreuschPaganAndKoenkerTest.sps file and wanted to add it in the Analyze/Regression tool of SPSS version 23 but when i go through the utillities/install local...

31 August 2020 4,603 8 View

Can excess doping in a p-type semiconductor produce enough internal electric field exhibit Franz-Keldysh effect leading to band tailing?

Can excess doping produce enough internal electric field in a p-type semiconductor to exhibit Franz-Keldysh effect leading to band tailing? What could be the nature of such internal electric...

30 August 2020 8,103 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View