Poonam Roshan

4 Questions 4 Answers 0 Followers

Questions related from Poonam Roshan

Is it necessary to bind purified protein on to membrane at 4Oc for protein overlay assay or can this step be performed at room temperature?

Purified protein is being used as probe and target proteins are bound on to membrane. can anyone share their experience in this context?

07 November 2014 6,591 2 View

What OD should be used for agrobaterium culture to be inoculted in plants?

Agrobacterium culture OD?

04 September 2013 3,738 4 View

Protein Purification

Protein is found to be present in supernatant and I am trying to purify it by means of his tag columns but I am getting multiple bands other than my protein of interest. Can anyone offer suggestions?

29 August 2012 6,368 10 View

How to obtain desired protein from a pellet, as the protein of interest is coming in the pellet?

Protein should be in its native form after extraction from pellet.

10 July 2012 1,947 1 View