How to modify DNA (genomic dna or plasmid dna, not primer oligues) 3' end so that it will not be extended by proofreading polymerase?

05 May 2018 0 6K Report

In a reaction i will mix dna fragments from different sources. But i need only one of my dna substrate get extended, not the others. so how to seal the 3' end of those dna?

And i am using proofreading polymerase, so the sealing should not be removed by the 3 to 5 exonuclease activity of the polymerase.

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