Xinglin Jiang

3 Questions 4 Answers 0 Followers

Questions related from Xinglin Jiang

In pcr, are the templates double stranded or single stranded at 72oC extension?

1) 72 oC is low enough for a long primer to bind to single stranded template, so it should not be a problem for the template to turn back to double stranded form (as it have even higher Tm). 2)...

03 March 2019 8,432 5 View

Does cell fusion really work for Gram negative bacteria?

There are only very limited number of papers that report the cell fusion (electrofusion, protoplast fusion or L form cell fusion) of gram negative bacteria, like E.coli. But non of them have...

01 January 2019 6,279 0 View

How to modify DNA (genomic dna or plasmid dna, not primer oligues) 3' end so that it will not be extended by proofreading polymerase?

In a reaction i will mix dna fragments from different sources. But i need only one of my dna substrate get extended, not the others. so how to seal the 3' end of those dna? And i am using...

05 May 2018 5,973 0 View