How to measure zinc content in bacterial culture supernatant?

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Experience on Data for Master Thesis on Disposition Effect in Social Trading Platforms?

Dear Forum, My master thesis investigates the disposition effect—where traders sell winning shares too early and hold onto losing shares too long—on social trading platforms (STPs) like eToro,...

08 July 2024 187 0 View

Assay cascades - how do they work in DMTA cyle?

Could you explain to me, how do assay cascades work during the DMTA (Design-Make-Test-Analyze) cycle of Drug Discovery? What is involed in it? How many assays are usually present?

24 June 2024 7,245 4 View

My conditional effects are significant but my moderation analysis is not, how come?

So I am doing my thesis on the relation between cyberbullying perpetration and life satisfaction and if physical activity is a moderator. But in my moderation analysis the interaction is...

17 June 2024 6,400 2 View

2 Research Gate profiles?

Dear ResearchGate, I seem to have 2 independent profiles on your site which I do not know how could happen - can you, please, merge those into one? Both are on my name, but affiliations, however,...

04 June 2024 5,682 0 View

Hi. Can I use a plasmid with CMV promoter in cell-free in vitro system?

I need to express some peptides in cell-free system (fe TnT) but my plasmid has CMV promoter (no T7 or Sp6).

21 May 2024 4,964 4 View

Induction check BL21 DE3?

Hello, I am currently trying to optimise a bacterial expression system using BL2 1DE3 bacteria and IPTG. In order to just have a quick look, whether I can see any protein overexpression on a...

04 May 2024 3,549 2 View

The right to pre-trial release in the context of the constitution?

criminal procedure

29 April 2024 1,705 2 View

Itc experiments procedure?

I'm a beginner in ITC, does anyone have an idea why when measuring the heat of dilution and in measuring the interaction of HSA with the drug or even with the buffer itself, the curve goes up? HSA...

28 April 2024 2,113 3 View

How do I prevent dark blue spots/smear and the running front of my BN-PAGE?

Hi everyone, we are trying to establish BN-gels in our lab and we are currently facing some problems. We would like to investigate protein-protein interaction of membrane bound proteins from cell...

18 April 2024 3,219 0 View

Semaphorin3A treatment: How to use semaphorine to repulse axons and improve dendritic harborization in my culture chamber?

Good morning, I need to isolate dendrites from axons in my microchamber, I have already tried a protocol of Netrin and it failed. I would like to put Sema3a in my dendritic compartment in order...

26 March 2024 2,632 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View