8 Questions 15 Answers 0 Followers
Questions related from Anna Pedroncelli
Hello, I need to extract DNA from leaf and root tissues using the DNeasy Plant Mini Kit (Qiagen). Due to the large number of samples, I have stored both leaves and roots at -20 °C until...
05 June 2025 3,681 4 View
I was told there should be a summary table in the genome overview, but there is not and the support did not reply to my email.
15 February 2025 2,312 0 View
Hi! I have a bacterial strain that changes the pH of the broth making it acidic. I want to get a knock-out mutant that is unable to lower the pH. Since I'm using transposon mutagenesis without...
15 January 2024 539 3 View
24 October 2023 4,690 0 View
Hi everyone! I'm trying to amplify a gene 900 bp long. I have the "right" band, but also a band with a higher length, under ~2000 bp. Once I purified the 900 bp band from gel, I ran the...
06 March 2023 8,817 4 View
Hi everyone! I would like to insert a plasmid (containing genes for resistance to Gentamycin and Chloramphenicol) into an Erwinia amylovora strain resistant to Rifampicin. So far, I've tried three...
19 September 2022 4,139 5 View
Hi everyone! I am using the Mauve Contig Mover to figure out the exact position of the draft genome contigs on the reference genome. The point is that it's not so easy to link the information...
31 August 2022 6,315 0 View
Hi everyone! I would like to measure the zinc content of a bacterial culture supernatant using the spectrophotometer. Any suggestions? Thanks! Anna
19 July 2022 7,552 10 View
