Anna Pedroncelli

5 Questions 12 Answers 0 Followers

Questions related from Anna Pedroncelli

How can I find bacterial genes involved in the acidification of the growth medium?

Hi! I have a bacterial strain that changes the pH of the broth making it acidic. I want to get a knock-out mutant that is unable to lower the pH. Since I'm using transposon mutagenesis without...

24 October 2023 4,650 0 View

PCR non-desired band: Is it possible to get a ~2000 bp with an elongation time set for a fragment 900 bp long? ?

Hi everyone! I'm trying to amplify a gene 900 bp long. I have the "right" band, but also a band with a higher length, under ~2000 bp. Once I purified the 900 bp band from gel, I ran the...

06 March 2023 8,782 4 View

Erwinia amylovora transformation method?

Hi everyone! I would like to insert a plasmid (containing genes for resistance to Gentamycin and Chloramphenicol) into an Erwinia amylovora strain resistant to Rifampicin. So far, I've tried three...

19 September 2022 4,088 5 View

How to mapping the contigs of a draft genome on the reference genome?

Hi everyone! I am using the Mauve Contig Mover to figure out the exact position of the draft genome contigs on the reference genome. The point is that it's not so easy to link the information...

31 August 2022 6,282 0 View

How to measure zinc content in bacterial culture supernatant?

Hi everyone! I would like to measure the zinc content of a bacterial culture supernatant using the spectrophotometer. Any suggestions? Thanks! Anna

19 July 2022 7,487 10 View