May I know how to run BAPS software for genetic structure analysis?

I converted my genepop file into BAPS file using CREATE. I have read the manual yet unable to understand how to proceed further. Any recommendations would be helpful.

20 April 2020 6,166 1 View

In sandwich ELISA, whether the coating antibody and primary antibody should be raised from different species or should belong to the same species?

Suppose the coating antibody (may be against some pathogen affecting chicken, for instance Newcastle disease virus i.e anti-NDV IgG from chicken) used in the sandwich ELISA is raised in chickens,...

12 December 2017 3,844 3 View

How to prevent the precipitation of starch in media by ammonium sulphate precipitation?

My media contains starch (1% w/v) in LB. But the problem is that the starch is also getting precipitated by during ammonium sulphate precipitation (60% w/v). Can anyone suggest how to prevent the...

08 June 2014 1,400 4 View

How can I control alkaline pH >12 during growth of A.baumanii for cutinase production?

I use LB media supplemented with cutin, but the pH of the media goes beyond 12 which creates problems for P-nitrophenyl butyrate assay, since pNPB is not stable under alkaline pH conditions (> pH...

22 May 2014 3,567 3 View

Can anyone suggest simple methods for preparation of reference electrodes?

I am planning for preparation of a home-made Ag/AgCl reference electrode. Can anyone suggest how to prepare and calibrate the electrode?

12 August 2012 7,746 12 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View

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23 February 2021 4,014 3 View

How to find natural frequency of a bridge from vehicle induced vibrations?

i have frequencies of bridge that are effected by mass of vehicle. the problem is when any vehicle pass the bridge it would add the its mass to the bridge mass ultimately mass of the bridge will...

22 February 2021 10,004 3 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View