How many mouse splenocytes were stored in each cryovials?

I am planning to cryopreserved mice splenocytes in 10% DMSO +90% FBS media ( 1ml is added in each vial) for future experiments. I am NOT sure what's minimum number of mice splenocytes cells should...

03 December 2018 5,771 1 View

In sandwich ELISA, whether the coating antibody and primary antibody should be raised from different species or should belong to the same species?

Suppose the coating antibody (may be against some pathogen affecting chicken, for instance Newcastle disease virus i.e anti-NDV IgG from chicken) used in the sandwich ELISA is raised in chickens,...

12 December 2017 3,844 3 View

Can we perform Flow cytometry based NK cell cytotoxicity assay using melanoma cell line (B16F10)?

I did Flow Cytometry based NK cell cytotoxicity assay using anti-CD71 targets K562 and U266 cell line. Now I want to repeat same experiment using melanoma cell line (B16F10). Would you please...

06 December 2017 1,724 3 View

define Cloud Computing and Data Masking Techniques?

hi anyone working on data masking techniques?

27 November 2017 4,103 3 View

In WSN,is it necessary for nodes to receive all packets broadcasted by a node lies in their periphery?

19 October 2016 2,534 4 View

Why the saturated magnetization decreases when iron nanoparticle is coated with Chitosan and increases when coated with CTAB?

14 October 2016 3,647 1 View

Which algorithm is better than hough transform for edge detection?

edge detection algorithm in image processing

29 February 2016 729 8 View

Can anyone suggest how to calculate the concentration of the compound through peak area and RT using GCMS for FAME?

27 March 2015 8,137 10 View

How can I carry out protein purification using D-salt dextran desalting column?

I need to get purified a 10 mL protein sample using D-salt dextran desalting column, for my research work. Can anyone suggest me some company or organization dealing with protein purification in...

29 July 2014 2,894 2 View

How to prevent the precipitation of starch in media by ammonium sulphate precipitation?

My media contains starch (1% w/v) in LB. But the problem is that the starch is also getting precipitated by during ammonium sulphate precipitation (60% w/v). Can anyone suggest how to prevent the...

08 June 2014 1,400 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View