May I know how to run BAPS software for genetic structure analysis?

I converted my genepop file into BAPS file using CREATE. I have read the manual yet unable to understand how to proceed further. Any recommendations would be helpful.

20 April 2020 6,166 1 View

How many mouse splenocytes were stored in each cryovials?

I am planning to cryopreserved mice splenocytes in 10% DMSO +90% FBS media ( 1ml is added in each vial) for future experiments. I am NOT sure what's minimum number of mice splenocytes cells should...

03 December 2018 5,771 1 View

In sandwich ELISA, whether the coating antibody and primary antibody should be raised from different species or should belong to the same species?

Suppose the coating antibody (may be against some pathogen affecting chicken, for instance Newcastle disease virus i.e anti-NDV IgG from chicken) used in the sandwich ELISA is raised in chickens,...

12 December 2017 3,844 3 View

Can we perform Flow cytometry based NK cell cytotoxicity assay using melanoma cell line (B16F10)?

I did Flow Cytometry based NK cell cytotoxicity assay using anti-CD71 targets K562 and U266 cell line. Now I want to repeat same experiment using melanoma cell line (B16F10). Would you please...

06 December 2017 1,724 3 View

define Cloud Computing and Data Masking Techniques?

hi anyone working on data masking techniques?

27 November 2017 4,103 3 View

In WSN,is it necessary for nodes to receive all packets broadcasted by a node lies in their periphery?

19 October 2016 2,534 4 View

Why the saturated magnetization decreases when iron nanoparticle is coated with Chitosan and increases when coated with CTAB?

14 October 2016 3,647 1 View

Which algorithm is better than hough transform for edge detection?

edge detection algorithm in image processing

29 February 2016 729 8 View

Can anyone suggest how to calculate the concentration of the compound through peak area and RT using GCMS for FAME?

27 March 2015 8,137 10 View

How can I carry out protein purification using D-salt dextran desalting column?

I need to get purified a 10 mL protein sample using D-salt dextran desalting column, for my research work. Can anyone suggest me some company or organization dealing with protein purification in...

29 July 2014 2,894 2 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to classify strictly upper triangular matrices by similarity?

Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

01 March 2021 2,957 8 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View