I am planing to measure total protein concentration of chicken amniotic fluid by using pierce bca protein assay kit.
Am i able to detect the correct range of concentration by using the kit. the kit working range is from 20-2000 ug/mL.
Also, I would like to use fetal bovine serum as a control. As FBS is very concentrated with proteins, would it able to measure in that working range? or the serum conc would be too high?
For measuring the total protein concentration of a sample, Bradford assay could be the best option as well. With just Bradford reagent and bovine serum albumin (used as standard), you can determine the total protein concentration.
You don't have to find a kit that include your protein concentration in it's working range. You simply dilute your sample until they are in the working range of the kit you are using.
In my opinion, you don't need a Bradford. BCA is a good method for determining protein concentration. It react to a broad range of protein in a similar matter, is somewhat resistant to detergent and is fast enough. I'm not sure what aminiotic fluid contains but you can read this for a quick pro and cons of each method: https://advansta.com/bradford-vs-bca-protein-assay-camp/
You can try to find a paper that reference the concentration of your particular specie or if you can't find it, do a serial dilution with a non-precious sample and see how much you have to dilute your sample so that they can fit on your curve. That will give you an idea how much dilution you will need to use for your real experiment.
I agree with Jean. BCA complexes with peptide bonds and is a good overall protein assay.
But you need to do serial dilutions of your samples to be in the linear range of your positive control (usually BSA).
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