9 Questions 1 Answers 0 Followers
Questions related from Oxford Leong
I am working on the field of cell culture and related to the alternative of cell culture media. And I would like to publish my research finding/article. May I know which journal should I publish...
18 November 2018 6,958 5 View
11 November 2018 1,754 5 View
Unfortunately, the highest wavelength in my lab's spectrophotometer is 492nm with a reference wavelength of 620 nm. From what I read, most published paper used 570nm wavelength to measure their...
08 August 2018 1,222 4 View
I was using MDCK cells to grow Influenza H1N1 in 12-wells plates. Cells was seeded 1 x 10^6 cells/well and left overnight in media without serum. Cells were then infected with H1N1 at moi of 0.1...
08 August 2018 1,673 14 View
MTT (Methylthiazolyldiphenyl-tetrazolium bromide) sigma cat. no. M2128 I am going to reconstitute MTT with Phosphate buffered solution at 5 mg/mL. Can I store the reconstituted solution in -20C...
08 August 2018 3,629 0 View
I have bought ovotransferrin/conalbumin which is in the powder form. The recommended storage temperature is -20C. What solvent can I use to dissolve and use it for cell culture purpose? Can I...
27 July 2018 809 1 View
I am planing to buy some proteins for my experiment, either in solution or in lyophilised powder form. I am confused with what those store website (e.g. sigma) has shown for the packaging size....
07 July 2018 4,760 1 View
07 July 2018 2,415 1 View
BSA: Bovine serum albumin I would like to grow my cells in DMEM without serum but with BSA. There are two forms of protein supplement (e.g. BSA) in the market, one is lyophilised powder and one is...
07 July 2018 3,742 5 View